A Procedure for Immobilizing Enzymes on Nylon

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Abstract
One widely used procedure -for immobilizing enzymes on nylon was first described by D.L. Morris, et. al. in 1975 (Biochem. J., 147, 153). This report describes the optimization of this procedure, leading to a reduction in reaction time from 20 hours to 80 minutes and concomitant increase in the activity of the open-tubular reactors by as much as a factor of four. Type 66 nylon reactors holding three different enzymes, glucose oxidase, catalase, and urease, were prepared by the modified procedure and tested in a segmented continuous flow instrument.