B220: a B cell-specific member of the T200 glycoprotein family

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Abstract
T200, a major cell-surface glycoprotein on lymphoid cells, exists in several forms with different electrophoretic mobilities. These forms were correlated with different classes of lymphoid cell. The smaller forms, with MW .simeq. 170,000 and 180,000, are found predominantly on T cells; the 220,000 MW form is associated with B cells. The polypeptide portions of each molecule may be identical or closely related as all 3 forms share the same allelic variations; all reported heterologous antisera and monoclonal antibodies to T200 precipitate all 3 forms. A monoclonal antibody [derived from mouse myeloma S194/S.XXO.BU-1 cell rat spleen cell hybridoma] specific for the 220,000 MW form of T200 is reported. It is expressed only on B cells and a subset of bone marrow cells which includes B cell precursors. This form of the molecule was provisionally designated B220.