Characterization of glucagon‐expressing neurons in the chicken retina
- 29 March 2006
- journal article
- research article
- Published by Wiley in Journal of Comparative Neurology
- Vol. 496 (4) , 479-494
- https://doi.org/10.1002/cne.20937
Abstract
We recently identified large glucagon‐expressing neurons that densely ramify neurites in the peripheral edge of the retina and regulate the proliferation of progenitors in the circumferential marginal zone (CMZ) of the postnatal chicken eye (Fischer et al. [2005] J Neurosci 25:10157–10166). However, nothing is known about the transmitters and proteins that are expressed by the glucagon‐expressing neurons in the avian retina. We used antibodies to cell‐distinguishing markers to better characterize the different types of glucagon‐expressing neurons. We found that the large glucagon‐expressing neurons were immunoreactive for substance P, neurofilament, Pax6, AP2α, HuD, calretinin, trkB, and trkC. Colocalization of glucagon and substance P in the large glucagon‐expressing neurons indicates that these cells are the “bullwhip cells” that have been briefly described by Ehrlich et al. ([1987] J Comp Neurol 266:220–233). Similar to the bullwhip cells, the conventional glucagon‐expressing amacrine cells were immunoreactive for calretinin, HuD, Pax6, and AP2α. Unlike bullwhip cells, the conventional glucagon‐expressing amacrine cells were immunoreactive for GABA. While glucagon‐immunoreactive amacrine cells were negative for substance P in central regions of the retina, a subset of this type of amacrine cell was immunoreactive for substance P in far peripheral regions of the retina. An additional type of glucagon/substance P‐expressing neuron, resembling the bullwhip cells, was found in far peripheral and dorsal regions of the retina. Based on morphology, distribution within the retina, and histological markers, we conclude that there may be four different types of glucagon‐expressing neurons in the avian retina. J. Comp. Neurol. 496:479–494, 2006.Keywords
This publication has 60 references indexed in Scilit:
- Glucagon-Expressing Neurons within the Retina Regulate the Proliferation of Neural Progenitors in the Circumferential Marginal Zone of the Avian EyeJournal of Neuroscience, 2005
- Glucagon Receptor Agonists and Antagonists Affect the Growth of the Chick Eye: A Role for Glucagonergic Regulation of Emmetropization?Investigative Opthalmology & Visual Science, 2005
- Magnitude of Binocular Vision Controlled by Islet-2 Repression of a Genetic Program that Specifies Laterality of Retinal Axon PathfindingCell, 2004
- Homeostasis of Eye Growth and the Question of MyopiaPublished by Elsevier ,2004
- Retinal target cells of the centrifugal projection from the isthmo‐optic nucleusJournal of Comparative Neurology, 2004
- Nerve growth factor receptor TrkA is expressed by horizontal and amacrine cells during chicken retinal developmentJournal of Comparative Neurology, 1998
- Development of NADPH‐Diaphorase in the Avian Retina: Regulation by Calcium Ions and Relation to Nitric Oxide SynthaseJournal of Neurochemistry, 1996
- Trout and chicken proglucagon: alternative splicing generates mRNA transcripts encoding glucagon-like peptide 2Molecular Endocrinology, 1995
- Calbindin and calretinin localization in retina from different speciesVisual Neuroscience, 1990
- Distribution of substance P‐like immunoreactive retinal ganglion cells and their pattern of termination in the optic tectum of chick (Gallus gallus)Journal of Comparative Neurology, 1987