CDC25‐dependent induction of inositol 1,4,5‐trisphosphate and diacylglycerol in Saccharomyces cerevisiae by nitrogen
- 3 August 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 307 (3) , 249-252
- https://doi.org/10.1016/0014-5793(92)80688-d
Abstract
The addition of ammonium sulfate to starved yeast cells leads to a 3‐ to 4‐fold rapid increase of the second messengers inositol 1,4,5‐trisphosphate (IP3) and diacylglycerol (DAG), the products of phosphoinositide‐specific phospholipase C (PI‐PLC). This response is reduced by dissecting the RAS‐activating Cdc25 protein, and is completely abolished by the cdc25‐1 mutation even at permissive temperature. Starved cdc25‐1 mutant cells have a strongly reduced IP3 content, but an at least 10‐fold increased DAG level compared to the isogenic wild‐type strain. NH4 does not stimulate cAMP synthesis, and glucose does not induce IP3 and DAG. Our data suggest that the Cdc25 protein controls a nitrogen‐specific signalling pathway involving the effector PI‐PLC, in addition to the glucose‐induced activation of adenylyl cyclase (AC).Keywords
This publication has 25 references indexed in Scilit:
- Transmembrane Receptors and Intracellular Pathways that Control Cell ProliferationAnnual Review of Physiology, 1992
- Involvement of the CDC25 gene product in the signal transmission pathway of the glucose-induced RAS-mediated cAMP signal in the yeast Saccharomyces cerevisiaeMicrobiology, 1991
- The glucose-induced polyphosphoinositides turnover inSaccharomyces cerevisiaeis not dependent on the CDC25-RAS mediated signal transduction pathwayFEBS Letters, 1990
- Recent insights in phosphatidylinositol signalingPublished by Elsevier ,1990
- Development of a novel, Ins(1,4,5)P3-specific binding assay: Its use to determine the intracellular concentration of Ins(1,4,5)P3 in unstimulated and vasopressin-stimulated rat hepatocytesCellular Signalling, 1989
- PHOSPHOLIPID BIOSYNTHESIS IN YEASTAnnual Review of Biochemistry, 1989
- Glucose‐induced cAMP signaling in Saccharomyces cerevisiae is mediated by the CDC25 proteinFEBS Letters, 1989
- SCH9, a gene of Saccharomyces cerevisiae that encodes a protein distinct from, but functionally and structurally related to, cAMP-dependent protein kinase catalytic subunits.Genes & Development, 1988
- The S. cerevisiae CDC25 gene product regulates the RAS/adenylate cyclase pathwayCell, 1987
- Interaction of cAMP with the CDC25-mediated step in the cell cycle of budding yeastExperimental Cell Research, 1986