OESTROGENIC AND ANTI-OESTROGENIC EFFECTS OF OESTRIOL, 16-EPI-OESTRIOL, 2-METHOXYOESTRONE AND 2-HYDROXYOESTRADIOL-17β ON THE RETICULO-ENDOTHELIAL SYSTEM AND REPRODUCTIVE TRACT

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Abstract
The present investigation deals with the effects of oestriol, 16-epioestriol, 2-methoxyoestrone and 2-hydroxyoestradiol-17β, when administered alone or together with oestradiol-17β, on the phagocytic activity of the reticulo-endothelial system (RES) and on the reproductive tract in ovariectomized mice. Female mice (T.O. Swiss strain), weighing 20–25 g., were used. Five animals were used to assess the effects of each dose of a compound, or combination of compounds, and ten animals were used as controls. They were treated once daily for 4 days and phagocytic activity was assessed by the carbon clearance method (Nicol, Vernon-Roberts & Quantock, 1965) on the 5th day. The mice were classified as oestrogen-positive or oestrogen-negative depending on the absence or presence of leucocytes in the vaginal smears (Biggers & Claringbold, 1954). Oestradiol-17β was administered orally by stomach tube. Oestriol, 16-epi-oestriol, 2-methoxyoestrone and 2-hydroxyoestradiol-17β were given by s.c. injection, each dose being dissolved in 0·2 ml. arachis oil. Phagocytic

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