Insulin-like growth factor II mediates resveratrol stimulatory effect on cathepsin D in breast cancer cells
- 1 January 2006
- journal article
- research article
- Published by Taylor & Francis in Growth Factors
- Vol. 24 (1) , 79-87
- https://doi.org/10.1080/08977190500366068
Abstract
Cathepsin D (CD) is an enzyme that promotes breast cancer. CD is stored intracellularly; however, we demonstrated that IGF-II promotes CD secretion in estrogen receptor positive (ER+) breast cancer cells. We also showed that resveratrol (RSV) stimulates IGF-II in ER(+) breast cancer cells. Thus, we designed this study to determine whether RSV regulates CD in MCF-7, T47D (ER+) breast cancer cells as well as in Hs578t (cancer) and MCF-10A (normal) ER − cell lines. RSV (10− 6 M) increased CD and IGF-II secretion in ER+ but not ER − cells. RSV treatment (10− 4 M) inhibited CD in ER+ but not in ER − cells. Transfection of ER − cells with proIGF-II increased CD secretion. RSV (10− 6 M) modulates CD secretion through IGF-II while RSV (10− 4 M) inhibits CD in ER+ but not ER − cells. Regulation of CD by RSV represents a novel mechanism by which RSV may protect against breast cancer.Keywords
This publication has 32 references indexed in Scilit:
- Invasión de los ganglios axilares en el carcinoma mamario con receptores hormonales negativosMedicina Clinica, 2004
- Estrogenic effects of resveratrol in breast cancer cells expressing mutant and wild-type estrogen receptors: role of AF-1 and AF-2Published by Elsevier ,2004
- Resveratrol modulates the phosphoinositide 3‐kinase pathway through an estrogen receptor α‐dependent mechanism: Relevance in cell proliferationInternational Journal of Cancer, 2003
- Procathepsin D in breast cancer: What do we know? Effects of ribozymes and other inhibitorsCancer Gene Therapy, 2002
- Cathepsin-D affects multiple tumor progression steps in vivo: proliferation, angiogenesis and apoptosisOncogene, 2002
- A mutated cathepsin-D devoid of its catalytic activity stimulates the growth of cancer cellsOncogene, 2001
- Promoter Usage for Insulin-like Growth Factor-II in Cancerous and Benign Human Breast, Prostate, and Bladder Tissues, and Confirmation of a 10th ExonBiochemical and Biophysical Research Communications, 2000
- Reversal of Cathepsin D Routing Modulation in MCF-7 Breast Cancer Cells Expressing Antisense Insulin-Like Growth Factor II (IGF-II)Hormone and Metabolic Research, 1999
- The third IGF-II promoter specifies transcription of three transcripts out of five in human placentaMolecular Reproduction and Development, 1992
- Overexpression and hormonal regulation of pro-cathepsin D in mammary and endometrial cancerJournal of Steroid Biochemistry, 1989