In Search of α1-Microglobulin on the Lymphocyte Surface

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Abstract
α1-Microglobulin was found by immunofluorescence not to be associated with human lymphoid and nonlymphoid cell lines. No accumulation of α1-microglobulin was detected in culture media of these cell lines. A weak membrane fluorescence with anti-α1-microglobulin on peripheral lymphocytes could not be blocked by the purified protein. No release of α1-microglobulin into the growth medium was seen by normal cultured leukocytes. Treatment of normal lymphocytes, erythrocytes, and various cell lines with solubilization techniques did not yield any α1-microglobulin. α1-Microglobulin and protein HC display immunologic and biochemical identity. However, anti-protein HC stained almost all of the tested cell lines and normal lymphocytes. Blocking experiments with the purified protein were not successful. Antibodies reacting with a minor impurity (50 000 d) in the α1-microglobulin or protein HC preparations could be absorbed from anti-α1-microglobulin with normal leukocytes and a lymphoid cell line.

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