THE EFFECTS OF ANOXIA AND OF HEMORRHAGE UPON THE METABOLISM OF THE CEREBRAL CORTEX OF THE RAT

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Abstract
Reproducible initial rates of resp. in the absence of substrate, and uniform steady rates for at least 3 hrs. in the presence of glucose were obtained in slices of rat cerebral cortex when the medium contained Ca, the initial dry wt. of the tissue was the basis for the rates of O2 uptake, and the time of prepn. of the tissues was standardized. The rate of resp. of brain slices from normal rats was depressed to an increasing degree with duration of anoxia by incubation in nitrogen for 10, 15, 20, and 30 mins. If glucose was added just before or at the end of anaerobiosis the rate of resp. was increased, but not to normal. The rate of O2 uptake of brain slices in air was nearly the same as that in O2. The rates of O2 uptake of brain slices from rats in shock after acute blood loss, or in the terminal stages of prolonged shock, were not significantly below normal, except in one instance in which the animal was anesthetized with nembutal. Changes in brain ammonia, "amide", amino N, and lactic acid, before and after incubation, were unchanged. Hence no consistent effects of shock after hemorrhage on the metabolism of brain tissue were observed. The brain may be better protected from anoxia than other tissues, or there may be metabolic processes affected which were not measured.

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