Rescue of Influenza A Virus from Recombinant DNA
- 1 November 1999
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 73 (11) , 9679-9682
- https://doi.org/10.1128/jvi.73.11.9679-9682.1999
Abstract
We have rescued influenza A virus by transfection of 12 plasmids into Vero cells. The eight individual negative-sense genomic viral RNAs were transcribed from plasmids containing human RNA polymerase I promoter and hepatitis delta virus ribozyme sequences. The three influenza virus polymerase proteins and the nucleoprotein were expressed from protein expression plasmids. This plasmid-based reverse genetics technique facilitates the generation of recombinant influenza viruses containing specific mutations in their genes.Keywords
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