Monoclonal Antibodies to Bovine Somatotropin: Immunoadsorbent Reagents for Mammalian Somatotropins
- 1 January 1984
- journal article
- research article
- Published by Mary Ann Liebert Inc in Hybridoma
- Vol. 3 (2) , 151-162
- https://doi.org/10.1089/hyb.1984.3.151
Abstract
Twenty-nine stable hybridoma cell lines secreting monoclonal antibodies to bovine somatotropin (bST) have been produced and characterized. Five of the monoclonal antibodies bind porcine and human somatotropins as well as bST. One of these antibodies was used as a reagent in immunoadsorbent chromatography of recombinant bST or pituitary bST from cell extracts. Following chromatography, the bST preparations retained activity in a rabbit liver radioreceptor assay and in a radioimmunoassay. The immunoadsorbent reagent bound human and porcine somatotropins as well as bovine.This publication has 25 references indexed in Scilit:
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