THE DEVELOPMENT OF ANTISERA TO PROSTAGLANDINS B2 AND F2α AND THEIR ANALYSIS USING SOLID‐PHASE AND DOUBLE ANTIBODY RADIOIMMUNOASSAY METHODS
Open Access
- 1 December 1975
- journal article
- Published by Wiley in British Journal of Pharmacology
- Vol. 55 (4) , 503-514
- https://doi.org/10.1111/j.1476-5381.1975.tb07425.x
Abstract
A method for the production of highly substituted prostaglandin‐bovine serum albumin conjugates has been developed. Antisera to prostaglandins B2 and F2α were raised in rabbits immunized with prostaglandin‐bovine serum albumin conjugates. The antisera were assessed for specificity and sensitivity by the double antibody radioimmunoassay method and after they were covalently linked to powdered cellulose to form a ‘solid‐phase’ system. Solid phase radioimmunoassays were developed using conventional shaking and in the presence of sucrose which obviates the need for continuous mixing of the incubates.Keywords
This publication has 11 references indexed in Scilit:
- Radioimmunoassay Measurement of Prostaglandins E, A, and F in Human PlasmaJournal of Clinical Investigation, 1973
- Preparation of specific antiserum to prostaglandin AProstaglandins, 1972
- Radioimmunoassay of the A prostaglandinsProstaglandins, 1972
- Antigenic activity of prostaglandins: Specificities of prostaglandins E1, A1 and F2α antigen-antibody reactionsProstaglandins, 1972
- A METHOD FOR PRODUCING SPECIFIC ANTISERA WITH SMALL DOSES OF IMMUNOGENJournal of Clinical Endocrinology & Metabolism, 1971
- Specificities of prostaglandins B 1 , F 1 , and F 2 antigen-antibody reactions.1971
- Radioimmunoassay of the F ProstaglandinsJournal of Clinical Endocrinology & Metabolism, 1971
- Radioimmunoassay for ProstaglandinsScience, 1971
- RADIOIMMUNOASSAYS EMPLOYING IMMUNOSORBENTSActa Endocrinologica, 1969
- STEROID-PROTEIN CONJUGATES .2. PREPARATION AND CHARACTERIZATION OF CONJUGATES OF BOVINE SERUM ALBUMIN WITH PROGESTERONE, DEOXYCORTICOSTERONE, AND ESTRONE1959