Covalent complex of phenylalanyl‐tRNA synthetase with 4‐thiouridine‐substituted tRNAPhe gene transcript retains aminoacylation activity1
- 1 May 1998
- journal article
- Published by Wiley in FEBS Letters
- Vol. 427 (1) , 1-4
- https://doi.org/10.1016/s0014-5793(98)00398-6
Abstract
S4U‐containing transcripts of tRNAPhe gene have been prepared by complete substitution of 16 U residues or by random incorporation of s4U residues followed by affinity electrophoresis isolation of s4U‐monosubstituted tRNA transcripts. Both analogs have been cross‐linked to Thermus thermophilus phenylalanyl‐tRNA synthetase (PheRS) and the specificity of the cross‐linking has been demonstrated. Functional activity of the covalent complex of PheRS with the s4U‐monosubstituted transcript has been shown by aminoacylation of 60% of the enzyme‐cross‐linked tRNA. This is the first instance in which biological activity of aminoacyl‐tRNA synthetase and cross‐linked tRNA in a specific complex has been revealed.Keywords
This publication has 17 references indexed in Scilit:
- The crystal structure of phenylalanyl-tRNA synthetase from Thermus thermophilus complexed with cognate tRNAPheStructure, 1997
- Recognition of tRNAPhe by Phenylalanyl‐tRNA Synthetase of Thermus ThermophilusEuropean Journal of Biochemistry, 1995
- Footprinting of tRNAPhetranscripts fromThermus thermophilusHB8 with the homologues phenylalanyl-tRNA synthetase reveals a novel mode of interactionNucleic Acids Research, 1995
- Recombinant photoreactive tRNA molecules as probes for cross-linking studiesBiochimie, 1994
- Alterations at the 3′‐CCA end of Escherichia coli and Thermus thermophilus tRNAPhe do not abolish their acceptor activityFEBS Letters, 1994
- The 2.9 Å Crystal Structure of T. thermophilus Seryl-tRNA Synthetase Complexed with tRNA
Ser
Science, 1994
- Biochemical and physical characterization of an unmodified yeast phenylalanine transfer RNA transcribed in vitro.Proceedings of the National Academy of Sciences, 1988
- Phenylalanyl‐tRNA synthetase from Thermus thermophilus HB8 Purification and properties of the crystallizing enzymeFEBS Letters, 1988
- Isolation of specific tRNAs using an ionic-hydrophobic mixed-mode chromatographic matrixAnalytical Biochemistry, 1985
- Incorporation of 1,N6‐ethanoadenosine into the 3′ terminus of tRNA using T4 RNA ligase 1. Preparation of yeast tRNAPhe derivativesEuropean Journal of Biochemistry, 1984