Embedding Free-Floating Cells and Microscopic Particles: Serum Albumin Coagulum—Epoxy Resin

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Abstract
Small quantities of cells or microscopic particles are fixed in suspension, centrifuged at low speed in a conical tube and the supernatant fluid removed as completely as possible. The pellet is resuspended in 0. 25 ml of 2 [degree]/o bovine serum albumin (in 0. 05 [image] Tris buffer, pH 7. 5) and transferred to a small, conical cellulose tube (Beckman No. 303369). One drop of 25 [degree]/o glutaraldehyde is mixed with the suspension and the tube is quickly centrifuged in a swinging bucket rotor. A gel forms in about 5 min. and the coagulum can be cut and processed through embedding like small blocks of tissue.

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