Binding of misonidazole to hypoxic cells in monolayer and spheroid culture: Evidence that a side-chain label is bound as efficiently as a ring label

Open Access
Publisher Website
Google Scholar
Abstract
The binding of ring-labeled and side-chain labeled misonidazole to hypoxic cells in monolayer and spheroid cultures of mammalian cells was compared. The kinetics and patterns of binding for the 2 labeled compounds are indistinguishable. This finding has implications for the mechanism of binding and for the design of misonidazole analogs which might be used to identify hypoxic zones in tumors [a phenomenon of relevance to the success or failure of tumor radiotherapy].