Immunopathologic and Histologic Studies on Skin from Pemphigus Patients in Tissue Culture

Abstract
Explants of skin from patients with pemphigus vulgaris and pemphigus folicaceus taken in the active stages of the disease had in vivo bound Ig[immunoglobulin]G in the intercellular area. After 24-48 h incubation of these explants in normal sera acantholytic bullae developed above the stratum basale, thus indicating that the bound IgG is probably in vivo bound pemphigus antibody. In both cases, skin from the inactive stage of the disease contained no in vivo bound pemphigus antibodies. Explants of these skin specimens failed to develop acantholysis in culture.

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