METHYLCELLULOSE CULTURES CLOTTED AND STAINED FOR PERMANENT RECORD

  • 1 January 1980
    • journal article
    • research article
    • Vol. 8  (9) , 1081-1085
Abstract
Whereas methylcellulose cultures have been valuable for biochemical studies on hemopoiesis in vitro, it has been difficult to study cellular morphology in these cultures. This study demonstrates that clotting factors can be added at any time during the culture period and non-adherent [mouse] cells become entrapped in a semisolid clot. The clot can be removed, fixed and stained for detailed microscopic examination.