The role of SmpB protein in trans‐translation
Open Access
- 1 February 2002
- journal article
- Published by Wiley in FEBS Letters
- Vol. 514 (1) , 74-77
- https://doi.org/10.1016/s0014-5793(02)02333-5
Abstract
The function of SmpB protein in the trans‐translation system was evaluated using the well‐defined cell‐free translation system consisting of purified ribosome, alanyl‐tRNA synthetase and elongation factors. The analysis showed that SmpB protein enhances alanine‐accepting activity of tmRNA and that SmpB protein and tmRNA are sufficient to complete the trans‐translation process in the presence of translational components. Moreover, SmpB is indispensable in the addition of tag‐peptide onto ribosomes by tmRNA. In particular, the A‐site binding of tmRNA is inhibited in the absence of SmpB.Keywords
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