Centrifugal analyzer method for total bilirubin in serum by use of diazotized 2-chloroaniline-5-sulfonic acid.
Open Access
- 1 February 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 25 (2) , 205-208
- https://doi.org/10.1093/clinchem/25.2.205
Abstract
We describe two centrifugal analyzer methods for measuring total bilirubin in serum. Diazotized 2-chloroaniline-5-sulfonic acid is used in both. In the first procedure, ethylene glycol and methanol are used as an accelerator solvent; results correlate well with those by a Jendrassik and Grof method adapted to the centrifugal analyzer, but there is considerable hemolysis interference. In the second method, dimethyl sulfoxide is used with the ethylene glycol/methanol solvent and almost all hemolysis interference is eliminated. For either method, a 15-microliter sample is required. In the second method, instrument response is linearly related to concentration to 250 mg/L and the within-run precision (CV) is about 1%.This publication has 2 references indexed in Scilit:
- Kinetics of the reactions of unconjugated and conjugated bilirubins with p-diazobenzenesulfonic acid.Clinical Chemistry, 1978
- Characteristics of statistical parameters used to interpret least-squares results.Clinical Chemistry, 1978