Subcellular Localization of Two Long‐Chain Acyl‐Coenzyme‐A Synthetases in Candida lipolytica

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Abstract
Studies were made on the subcellular localization of 2 long-chain acyl-coenzyme-A synthetases as well as glycerolphosphate acyltransferase and the acyl-CoA-oxidizing system in C. lipolytica grown on oleic acid. Acyl-CoA synthetase I is distributed among different subcellular fractions, including microsomes and mitochondria where glycerolphosphate acyltransferase is located. Acyl-CoA synthetase II is localized in microbodies where the acyl-CoA-oxidizing system is located. These results support the previous conclusion that acyl-CoA synthetase I is responsible for the production of acyl-CoA to be utilized for the synthesis of cellular lipids, while acyl-CoA synthetase II provides acyl-CoA that is exclusively degraded via .beta.-oxidation.