INVITRO STIMULATION BY MITOGENS OF PERIPHERAL-BLOOD LYMPHOCYTES FROM RAINBOW-TROUT (SALMO-GAIRDNERI)

  • 1 January 1978
    • journal article
    • research article
    • Vol. C129  (1) , 3-12
Abstract
Peripheral blood lymphocytes of the rainbow trout can be stimulated by PHA [phytohemagglutinin], con A [concanavalin A] and LPS [lipopolysaccharide]. Optimal concentrations for stimulation are 1-50 .mu.g/ml for PHA, 1-10 .mu.g/ml for conA and 1 .mu.g/ml for LPS. Best stimulation is obtained after an incubation period of 72 h at a temperature of 28.degree. C. Thymidine incorporation into lymphocytes is enhanced when PHA and LPS (1 .mu.g/ml each) are given simultaneously. After the 1st stimulation by LPS the lymphocytes become less sensitive to a 2nd exposure to this same mitogen. LPS-primed lymphocytes are highly reactive to PHA. There may be (at least) 2 populations of peripheral blood lymphocytes, 1 sensitive to PHA and the other sensitive to LPS.