Immunoassay Studies Using Adenosine Deaminase Enzyme with Potentiometric Rate Measurement
- 3 January 1981
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 14 (2) , 97-109
- https://doi.org/10.1080/00032718108081385
Abstract
An enzyme immunoassay method using adenosine deaminase (EC 3.5.4.4) as the enzyme label is described. Potentiometric rate measurements with an ammonia gas-sensing electrode were used to determine the activity of enzyme label bound to agarose bead immobilized 2nd antibody. The activity is related to the concentration of either a model haptenic group dinitrophenyl, DNP, or [rabbit] anti-DNP antibody. Detection limits of 50 ng antibody and 10-10 M DNP hapten were obtained when incubations were carried out at pH 7.5 and assays at pH 9.0.This publication has 12 references indexed in Scilit:
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