LEUCOCYTE MIGRATION INHIBITION IN VITRO WITH INHIBITORS OF ASPARTIC AND SULPHHYDRYL PROTEINASES

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Abstract
The human leucocyte migration in the leucocyte migration under agarose technique (LMAT) was investigated with specific inhibitors of aspartic, sulphhydryl. metallo- and serine proteinases. The general aspartic proteinase inhibitor pepstatin and the highly specific competitive cathepsin D inhibitor, N-gly-glu-gly-phe-leu-gly-D-phe-leu suppressed leucocyte migration at concentrations of 20–200 μmol/l and 30–59 μmol/l, respectively. The suphhydryl enzyme inactivator, N-ethylmaleimide suppressed leucocyte migration at concentrations of 100–200 μmol/l. Several inhibitors of serine- and metallo enzymes were tested, but none had any effect on leucocyte mobility, although the metal binding agent 8-hydroxyquinoline was strongly inhibitory to cell migration, the significance of which is discussed.