STUDIES ON POLYNUCLEOTIDE-STIMULATED AMINO ACYL TRANSFER FROM SOLUBLE-RNA TO RAT LIVER RIBOSOMES
- 1 January 1963
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 49 (1) , 82-88
- https://doi.org/10.1073/pnas.49.1.82
Abstract
A rat liver system that catalyzes amino acyl transfer from amino acyl s-RNA to ribosomal protein is stimulated by the addition of polyuridylic acid. The stimulation is markedly greater when ribosomes are preincubated with pH 5 supernatant, particularly in experiments with low concentrations of ribosomes. When amino acyl transfer is examined with several polynucleotide preparations, varying in base composition, the incorporation of individual s-RNA-bound amino acids appears to be enhanced in the presence of specific polynucleotides. Partial characterization of this polynucleotide-stimulated mammalian system has been carried out. The results suggest that on preincubation, a component of ribosomes, possibly messenger RNA, is removed by a soluble factor which is different from the amino acyl transferring enzymes. The stimulation observed on addition of polynucleotides to preincubated ribosomes suggests that the natural messenger RNA is replaced by these synthetic messenger molecules.Keywords
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