Respiratory burst activity in brain macrophages: a flow cytometric study on cultured rat microglia
- 1 June 1991
- journal article
- Published by Wiley in Neuropathology and Applied Neurobiology
- Vol. 17 (3) , 223-230
- https://doi.org/10.1111/j.1365-2990.1991.tb00718.x
Abstract
A new flow cytometric method for the investigation of the respiratory burst of macrophages/ microglia isolated from neonatal rat brain has been established. Respiratory burst activity was measured quantitatively in single viable cells by the intracellular oxidation of non-fluorescent dihydrorhodamine 123 (DHR) to fluorescent rhodamine 123. Cultured microglia exhibited high spontaneous respiratory burst activity already before stimulation. After maximal stimulation with phorbol myristate acetate, DHR oxidation rose by 40–95%. The respiratory burst activity in resident or inflammatory, i.e. thioglycolate elicited, peritoneal macrophages was significantly lower than in cultured brain macrophages suggesting a high potential of microglia for oxidative tissue destruction.Keywords
This publication has 23 references indexed in Scilit:
- The macrophage in the persistence and pathogenesis of HIV infectionAIDS, 1989
- Macrophages and microglia in the nervous systemTrends in Neurosciences, 1988
- Functional plasticity of microglia: A reviewGlia, 1988
- Macroglia: neural cells responsive to lymphokines and growth factorsImmunology Today, 1987
- Production of superoxide anions by a CNS macrophage, the microgliaFEBS Letters, 1987
- Synthesis and biological studies of iodinated (127/125I) derivatives of rhodamine 123Journal of Medicinal Chemistry, 1987
- Ameboid microglia as effectors of inflammation in the central nervous systemJournal of Neuroscience Research, 1987
- Antigen presentation and tumor cytotoxicity by interferon‐γ‐treated microglial cellsEuropean Journal of Immunology, 1987
- Immunohistological analysis of macrophages in the central nervous system of Lewis rats with acute experimental allergic encephalomyelitisJournal of Neuroimmunology, 1986
- Interferon-γ induces the expression of H-2 and Ia antigens on brain cellsJournal of Neuroimmunology, 1985