A Selected Ion Monitoring GC/MS Assay for 3,4,4'-Trichlorocarbanilide and its Metabolites in Biological Fluids

Abstract
A selected ion monitoring gas chromatography/mass spectrometric method for the quantitative determination of 3,4,4′-trichlorocarbanilide (TCC) and its major metabolites (the 2′-hydroxy sulfate and the N- and N′-glucuronides) in human plasma and urine was developed using the deuterium-labelled compounds as internal standards. Limits of detection of 3 ng/mL in urine for the N-glucuronides and of 1.5 ng/mL in plasma for the 2′-hydroxy sulfate were attained. Use of the method was illustrated in a study in human subjects employing TCC-containing bar soaps.