Preferential uptake of lipids by mycoplasma membranes from human plasma low-density lipoproteins
- 7 December 1982
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 21 (25) , 6477-6482
- https://doi.org/10.1021/bi00268a024
Abstract
The binding and transfer of low-density lipoprotein (LDL) constituents to mycoplasma membranes were examined. Mycoplasma capricolum bound more 125I-labeled LDL than Acholeplasma laidlawii. Free and esterified cholesterol uptake was 3-4 times higher in M. capricolum than in A. laidlawii. Cholesterol transfer to the membranes of both organisms far exceeded the amounts of cholesterol expected according to LDL protein found associated with the membranes. Trypsin digestion of the membranes prior to incubation with LDL decreased the binding of LDL and the transfer of free cholesterol to M. capricolum membranes but did not affect these processes with A. laidlawii membranes. These findings suggest the existence of protease-sensitive receptors on M. capricolum cell surface responsible for tighter contact with LDL. Analysis of LDL after incubation with large amounts of M. capricolum membranes revealed a loss of 60% of the lipoprotein-free cholesterol, 25.9% of the esterified cholesterol, and 20.7% of phospholipids without appreciable loss of protein. Rate zonal ultracentrifugation showed that the LDL separated as a single symmetrical peak, denser than the control. EM showed that the lipoprotein particles retained their spherical shape but became about 10% smaller in diameter. Both free and esterified cholesterols are apparently transferable from LDL to the membranes by a simple exchange process which does not involve prolonged contact or fusion of the particle with the membrane and does not result in degradation of the lipoprotein particle. Similar mechanisms of free and esterified cholesterol transfer may also operate in vivo and contribute to the process of cholesterol exit from the plasma.This publication has 23 references indexed in Scilit:
- Cholesterol distribution and movement in the Mycoplasma gallisepticum cell membraneBiochemistry, 1978
- Metabolism of cholesterol-rich chylomicroms. Mechanism of binding and uptake of cholesteryl esters by the vascular bed of the perfused rat heart.Journal of Clinical Investigation, 1978
- Low Density LipoproteinJournal of Clinical Investigation, 1978
- Stimulation of esterified cholesterol accumulation in tissue culture cells exposed to high density lipoproteins enriched in free cholesterol.Journal of Lipid Research, 1978
- Cholesterol transfer from serum lipoproteins to mycoplasma membranesBiochemistry, 1977
- Degradation of cationized low density lipoprotein and regulation of cholesterol metabolism in homozygous familial hypercholesterolemia fibroblasts.Proceedings of the National Academy of Sciences, 1976
- Serum lipoproteins as cholesterol donors to mycoplasma membranesBiochemical and Biophysical Research Communications, 1976
- [10] Assay of inorganic phosphate, total phosphate and phosphatasesPublished by Elsevier ,1966
- THE DISTRIBUTION AND CHEMICAL COMPOSITION OF ULTRACENTRIFUGALLY SEPARATED LIPOPROTEINS IN HUMAN SERUMJournal of Clinical Investigation, 1955
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951