Regulatory sequences required for hst‐1 expression in embryonal carcinoma cells

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Abstract
The hst‐1 gene, which is implicated in mammalian embryonic development and morphological transformation of NIH3T3 cells, is expressed in undifferentiated F9 cells, but not in differentiated F9 and other well‐differentiated cells, such as PYS‐2, NIH3T3 and HeLa cells. An octamer element present in the 3' untranslated region acts as an enhancer. Although Oct3 is down‐regulated when F9 cells are differentiated, transient expression of Oct3 did not enhance the hst‐1 promoter activity in HeLa, NIH3T3 or PYS‐2 cells. Thus, the role of Oct3 on hst‐1 expression remains elusive, and an additional transcription factor which interacts may regulate hst‐1 transcription in association with Oct1, Oct3 or both.