The action of cytochalasin A on the in vitro polymerization of brain tubulin and muscle G‐actin
- 1 January 1976
- journal article
- research article
- Published by Wiley in Journal of Supramolecular Structure
- Vol. 5 (1) , 81-90
- https://doi.org/10.1002/jss.400050109
Abstract
The presence of cytochalasin A inhibits the self-assembly of beef brain tubulin and rabbit muscle G-actin in vitro and also decreases the colchicine binding of tubulin. Prior reaction of cytochalasin A with 2-mercaptoethanol destroys its inhibitory effects. It is shown that cytochalasin A exerts its actions by reacting with sulfhydryl groups, possibly causing irreversible structural changes in the proteins. Cytochalasin B does not affect the tubulin assembly reaction.Keywords
This publication has 26 references indexed in Scilit:
- Cytochalasin a inhibits the invitro polymerization of brain tubulin and muscle actinBiochemical and Biophysical Research Communications, 1976
- THE ROLE OF RINGS IN THE ASSEMBLY OF MICROTUBULES IN VITRO*Annals of the New York Academy of Sciences, 1975
- PURIFICATION OF TUBULIN AND ASSOCIATED HIGH MOLECULAR WEIGHT PROTEINS FROM PORCINE BRAIN AND CHARACTERIZATION OF MICROTUBULE ASSEMBLY IN VITRO*Annals of the New York Academy of Sciences, 1975
- THE ACTION OF ANTIBIOTICS ON ENZYME SECRETION IN YEASTS: STUDIES WITH CYTOCHALASIN A*Annals of the New York Academy of Sciences, 1974
- Assembly of microtubules from preformed, ring‐shaped protofilaments and 6‐s tubulinJournal of Supramolecular Structure, 1974
- The role of ring aggregates and other structures in the assembly of microtubulesJournal of Supramolecular Structure, 1974
- Regulation of size and birefringence of the in vivo mitotic apparatusJournal of Supramolecular Structure, 1974
- The Cytochalasans: A New Class of Biologically Active Microbial MetabolitesAngewandte Chemie International Edition in English, 1973
- Microfilaments in Cellular and Developmental ProcessesScience, 1971
- Effects of Cytochalasins on Mammalian CellsNature, 1967