Inhibition of human cytomegalovirus major immediate early gene expression by antisense RNA expression vectors

1 September 1993

journal article
Published by Microbiology Society in Journal of General Virology

Vol. 74 (9) , 1965-1967
https://doi.org/10.1099/0022-1317-74-9-1965

Abstract

We have used antisense oligonucleotides and expression vectors to inhibit human cytomegalovirus (HCMV) major immediate early (IE) gene expression. We find that oligonucleotides complementary to the HCMV 72K IE protein (IE1) coding region do inhibit HCMV infection, but this is non-specific. However, the use of certain antisense expression vectors, which express short oligonucleotides complementary to IE1, specifically inhibits IE1 expression at the protein level after introduction of IE expression vectors into cells or after HCMV infection.

Keywords

GENE EXPRESSION
PROTEIN
ANTISENSE
EXPRESSION VECTORS
INTRODUCTION
72K
HUMAN CYTOMEGALOVIRUS
IMMEDIATE EARLY

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