Effect of Certain Metabolic Inhibitors on Translocation of P32 in Bean Plants.

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Abstract
Studies were initiated in an effort to isolate the phases of metabolism most closely associated with the transport mechanism. The basic technique utilized was to apply a p32 soln. to one primary leaf blade of a bean (Phaseolus vulgaris var. Black Valentine) plant and subsequently measure the amt. of the material which had moved through the petiole of the treated leaf and into the stem. Various chemicals which might be expected to influence the metabolism of plant cells were injected into the treated leaf petioles. The translocation of P32 was inhibited when dinitrophenol or sodium fluoride was placed in the vicinity of the phloem cells through which the transport occurred. There appeared to be little or no effect on translocation of p32 from treatments with sodium fluoroacetate, 2,4-dichlorophenoxyacetic acid, indoleacetic acid, or triiodobenzoic acid. The results of treatments with sodium arsenite were not conclusive.