Modified Fluorescent Technique, Using Rhodamine, for Studies of Rhizobium japonicum -Soybean Symbiosis

Abstract
Rhodamine-conjugated capsular polysaccharides isolated from Rhizobium japonicum 61A76NS were used to examine binding between the labeled polysaccharides and soybean roots. Fluorescein-labeled polysaccharides were not satisfactory because soybean root hairs autofluoresce in the fluorescein region.