The Determination By Means of Capillary Gas Chromatography of the Benzodiazepines-Clobazam, Clonazepam, Diazepam, and Nitrazepam-and Their Principal Metabolites Currently Used In the Treatment of Epilepsy

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Abstract
The authors developed a gas chromatographic method allowing the quantification of those benzodiazepines in current use in the treatment of epilepsy, name ly clobazam, clonazepam, diazepam and nitrazepam. This method involved a butyl acetate extraction from plasma, and an analysis on a CP-Sil 5 WSCOT capillary column with electron-capture detection. Intra-day precision and accuracy were better than 10% for each of the compounds and their main metabolites, N-desmethyldiazepam, oxazepam, N-desraethylclobazam. the quantification limit was about 0.5 to 1 ng/ml for each compound. Linearity proved satisfactory between 1 and 4000 ng/ml. Adequate han previously. Besides, its sensitivity is sufficient for kinetic studies following a single dose administration.