Artificial Organelle: ATP Synthesis from Cellular Mimetic Polymersomes
Top Cited Papers
- 18 November 2005
- journal article
- letter
- Published by American Chemical Society (ACS) in Nano Letters
- Vol. 5 (12) , 2538-2542
- https://doi.org/10.1021/nl051896e
Abstract
A complex cellular process was reconstructed using a multiprotein polymersome system. ATP has been produced by coupled reactions between bacteriorhodopsin, a light-driven transmembrane proton pump, and F0F1-ATP synthase motor protein, reconstituted in polymersomes. This indicates that ATP synthase maintained its ATP synthesis and therefore its motor activity in the artificial membranes. This hybrid proteopolymersome will have wide application in a number of fields ranging from the in vitro investigation of cellular metabolism to the synthesis of functional “smart” materials.Keywords
This publication has 24 references indexed in Scilit:
- Toward hybrid proteo-polymeric vesicles generating a photoinduced proton gradient for biofuel cellsNanotechnology, 2005
- Nanoreactors based on (polymerized) ABA-triblock copolymer vesiclesChemical Communications, 2000
- THE ATP SYNTHASE—A SPLENDID MOLECULAR MACHINEAnnual Review of Biochemistry, 1997
- ATP Synthesis by the F0F1-ATPase from the Thermophilic Bacillus PS3 Co-reconstituted with Bacteriorhodopsin into LiposomesJournal of Biological Chemistry, 1995
- Partial separation of inwardly pumping and outwardly pumping bacteriorhodopsin reconstituted liposomes by gel filtrationFEBS Letters, 1988
- Coupling of Proton Flux to the Hydrolysis and Synthesis of ATPAnnual Review of Biophysics and Bioengineering, 1983
- Bacteriorhodopsin in liposomes. II. Experimental evidence in support of a theoretical modelBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1979
- Bacteriorhodopsin and the purple membrane of halobacteriaBiochimica et Biophysica Acta (BBA) - Reviews on Bioenergetics, 1979
- Bioluminescence Assay: Principles and PracticePublished by Wiley ,1968
- Coupling of Phosphorylation to Electron and Hydrogen Transfer by a Chemi-Osmotic type of MechanismNature, 1961