STUDIES ON GUINEA-PIG MACROPHAGE-MIGRATION INHIBITORY FACTOR (MIF) .1. GLYCOPROTEIN NATURE AND NET CHARGE

  • 1 January 1979
    • journal article
    • research article
    • Vol. 37  (3) , 532-539
Abstract
Guinea pig macrophage migration inhibitory factor (MIF), obtained by the stimulation of sensitized lymph node cells with tuberculin PPD [purified protein derivative], was characterized as a glycoprotein by the following criteria: its activity is destroyed by 0.02 M sodium periodate; when MIF-containing culture fluids are subjected to precipitation by perchloric acid (final concentration 1 M), the inhibitory activity is recovered in the supernatant; and MIF binds to Sepharose-linked concanavalin A and can be eluted with methyl-.alpha.-D-glucopyranoside. When MIF-containing culture supernatants are fractionated by isoelectrofocusing, migration inhibitory activity is recovered in a fraction with an isoelectric point of 4.4-4.6.