Sequence of the nucleocapsid gene from murine coronavirus MHV-A59
Open Access
- 11 February 1983
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 11 (3) , 883-891
- https://doi.org/10.1093/nar/11.3.883
Abstract
The nucleotide sequence of the RNA encoding the nucleocapsid protein of coronavirus MHV-A59 has heen determined. Copy DNA was prepared from mRNA isolated from virally infected cells, fragmented and cloned in the phage vector Ml 3 mp8 for direct sequence determination. A sequence of 1817 nu-cleotides, adjacent to the viral poly-A tail, was obtained. It contains a single long open reading frame encoding a protein of mol. wt. 49660, which is enriched in basic residues.Keywords
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