Expression of thec-fgr related transcripts in epstein-barr virus-associated malignancies

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Abstract
The proto‐oncogene c‐fgr is expressed at high levels in cell lines derived from lymphomas which are infected with Epstein‐Barr virus (EBV) (Cheah et al., 1986). mRNA extracted directly from biopsies of EBV‐infected tissues was analyzed on Northern blots to determine if c‐fgr is expressed during lympho‐proliferations induced in vivo by EBV and in nasopharyngeal carcinoma (NPC), the epithelial malignancy associated with the virus. Elevated levels of c‐fgr expression were detected in all EBV‐positive lympho‐proliferations in vivo but not in cell lines established by EBV infection in vitro. This indicates that the induction of the c‐fgr proto‐oncogene is not an essential component of EBV‐induced transformation. Although no c‐fgr expression was detected in EBV‐positive or ‐negative epithelial cell lines, the 3.0‐kb c‐fgr mRNA was detected at low levels in mRNA obtained from NPC biopsy specimens. However, NPC tissue, after passage in nude mice (which eliminates infiltrating lymphoid and myeloid cells) did not contain the 3.0‐kb c‐fgr mRNA. The absence of expression of c‐fgr in the malignant epithelial cells infected with EBV contrasts with the elevated level of the proto‐oncogene in EBV‐infected lymphoma tissue and cell lines established from lymphomas. This suggests differences in the expression of cellular functions in EBV‐induced malignancies of these 2 distinct cell types.