Microassay for adenosine deaminase, the enzyme lacking in some forms of immunodeficiency, in mouse preimplantation embryos

Publisher Website
Google Scholar
Abstract
A highly sensitive biochemical microassay has been developed for adenosine deaminase (ADA; EC 3.5.4.4), the enzyme deficient in ∼20% of cases of severe combined immunodeficiency disease (SCID). The microassay is capable of detecting femtomolar amounts of reaction product in a single blastomere from a mouse 8-cell embryo and thus is sensitive enough to be considered for the possible prehnplantation diagnosis of SCID in human embryos.