Localization of Glycosphingolipids in Human Tissues by Immunofluorescence

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Abstract
Two glycosphingolipids, ceramide lactose and globoside, were isolated from human erythrocyte stroma. Antisera to these compounds were prepared in rabbits and characterized by complement fixation and hapten inhibition. Immunofluorescence studies of human kidney revealed that globoside was present in the plasma membrane and cytoplasm of epithelial cells of the proximal convoluted tubule. In the spleen both glycolipids were detected only in the plasma membrane of reticuloendothelial cells of the red pulp. Although glycosphingolipids are found in many organs, these studies suggest that they are present in appreciable quantities in only a few types of cells. This observation provides the first evidence that glycosphingolipids may occur within cells as well as in the plasma membrane.

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