Reintedness of archaebacterial RNA polymerase core subunits to their eubacterial and eultaryotic equivalents
- 1 January 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 16 (16) , 8113-8128
- https://doi.org/10.1093/nar/16.16.8113
Abstract
The sequence of the genes encoding the four largest subunits of the RNA polymerase of the archaebacterium Methanobacterium thermoautotrophicum was determined and putative translation signals were identified. The genes are more strongly homologous to eukaryotic than to eubacterial RNA polymerase genes. Analysis of the polypeptide sequences revealed colinearity of two pairs of adjacent archaebacterial genes encoding the B" and B'' or A and C genes, respectively, with two eubacterial and two eukaryotic genes each encoding the two largest RNA polymerase subunits. This difference in sequence organization is discussed in terms of gene fusion in the course of evolution. The degree of conservation is much higher between the archaebacterial and the eukaryotic polypeptides than between the archaebacterial and the eubacterial enzyme. Putative functional domains were identified in two of the subunits of the archaebacterial enzyme.Keywords
This publication has 6 references indexed in Scilit:
- Cloning and physical mapping of RNA polymerase genes from Methanobacterium thermoautotrophicum and comparison of homologies and gene orders with those of RNA polymerase genes from other methanogenic archaebacteriaJournal of Bacteriology, 1988
- Affinity Labelling of the Active Center of DNA-dependent RNA Polymerases within the Archaebacterial KingdomSystematic and Applied Microbiology, 1988
- Prokaryotic and eukaryotic RNA polymerases have homologous core subunits.Proceedings of the National Academy of Sciences, 1987
- Structural homology between different archaebacterial DNA-dependent RNA polymerases analyzed by immunological comparison of their componentsThe EMBO Journal, 1983
- The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primersGene, 1982
- The Primary Structure of Escherichia coli RNA PolymeraseEuropean Journal of Biochemistry, 1981