SYNERGISTIC EFFECTS OF RECOMBINANT TUMOR-NECROSIS-FACTOR AND INTERFERON-GAMMA ON RAT-THYROID CELL-GROWTH AND IA-ANTIGEN EXPRESSION

  • 1 February 1988
    • journal article
    • research article
    • Vol. 63  (2) , 285-289
Abstract
In this study, we have investigated the effects of tumour necrosis factor (TNF), interferon-gamma (IFN-.gamma.) and interleukin-1 (IL-1) on rat thyroid cells, using the continuously growing, differentiated FRTL5 cell line. No effect on cell growth was found with any cytokine alone, but the combination of TNF and IFN-.gamma. was cytostatic. These cells were not killed in a 18-hr assay for cytotoxicity, but prolonged incubation (4 days) with TNF and IFN-.gamma. produced cell detachment and death. Normal rat thyroid cells in primary culture were also susceptible to the combination of TNF and IFN-.gamma. for 4 days, but not 18 hr. In addition, IFN-.gamma. treatment induced class II (Ia) antigens on thyroid cells, and this was enhanced by TNF. TNF alone did not cause Ia expression on thyroid cells but did increase the constitutive expression of class I antigens. These results suggest that IFN-.gamma. and TNF, which are likely to be released by the lymphocytes and macrophages infiltrating the gland in thyroid autoimmunity, may synergize to impair cell growth and produce aberrant Ia antigen expression in thyroiditis. This could be an important mechanism for disease perpetuation.