Four-Factor Model Analysis of Thymidine Incorporation into Mouse DNA and the Mechanism of Radiation Effects

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Abstract
Quastler''s 4-factor model for DNA synthesis has been elaborated for the specific case of thymidine incorporation into mouse nuclei, and analyzed by analog computer stimulation. The model predicts a depression of labelled thymidine uptake by exogenous nonlabeled thymidine, and also provides an approach to the analysis of radiation effects on label incorporation. Autoradiograph grain count data from squash preparations of several mouse tissues, after a variety of thymidine doses and X-ray treatments, are in qualitative agreement with the pre- dictions of the model. Analysis in terms of model parameters suggests that irradiation depresses labeled thymidine uptake in intestinal crypt cells by increasing the effective thymidine triphosphate pool size rather than by interfering with DNA synthesis directly.

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