Rabbit heart fatty acid-binding protein. Isolation, characterization, and application of a monoclonal antibody.
- 1 October 1989
- journal article
- research article
- Published by Wolters Kluwer Health in Circulation Research
- Vol. 65 (4) , 981-988
- https://doi.org/10.1161/01.res.65.4.981
Abstract
A fatty acid-binding protein (FABP) was purified from rabbit heart and characterized with respect to size, isoelectric point, and tissue distribution. This protein was found in red muscle, diaphragm, and aorta, as well as in the heart. Amino acid composition of rabbit heart FABP differed only slightly from the human and rat proteins. Rabbit heart FABP was shown to bind two molecules of fatty acid. A monoclonal antibody was developed and used to demonstrate the feasibility of a one-step purification with affinity chromatography. Cross-reactivity was found between the human protein and the rabbit antibody, and an immunoassay was developed to human heart FABP. Levels of human heart FABP in the plasma of patients with acute myocardial infarction were significantly elevated (83 +/- 9 micrograms/ml) compared with patients with pulmonary edema (52 +/- 7 micrograms/ml) and normal volunteers (28 +/- 5 micrograms/ml; p less than 0.05, mean +/- SEM).This publication has 23 references indexed in Scilit:
- Circulating cardiac myosin light chains in patients with angina at rest: Identification of a high risk subgroupJournal of the American College of Cardiology, 1988
- Cloning and tissue distribution of rat heart fatty acid binding protein mRNA: identical forms in heart and skeletal muscleBiochemistry, 1987
- The Metabolic Significance of Mammalian Fatty-Acid-Binding Proteins: Abundant Proteins in Search of a FunctionAnnual Review of Nutrition, 1987
- Binding of acyl-CoA to liver fatty acid binding protein: effect on acyl-CoA synthesisBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1987
- Binding of lysophosphatidylcholine to the rat liver fatty acid binding proteinBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1986
- “Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein AAnalytical Biochemistry, 1981
- Fatty acid binding protein. Role in esterification of absorbed long chain fatty acid in rat intestine.Journal of Clinical Investigation, 1976
- The role of fatty acid binding protein on the metabolism of fatty acids in isolated rat hepatocytesBiochemical and Biophysical Research Communications, 1976
- Fatty Acid-Binding Protein in Small Intestine IDENTIFICATION, ISOLATION, AND EVIDENCE FOR ITS ROLE IN CELLULAR FATTY ACID TRANSPORTJournal of Clinical Investigation, 1974
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970