Multiphoton switching dynamics of single green fluorescent proteins
- 21 September 2004
- journal article
- research article
- Published by American Physical Society (APS) in Physical Review E
- Vol. 70 (3) , 030901
- https://doi.org/10.1103/physreve.70.030901
Abstract
Multi-photon driven photo-switching between dark and bright (fluorescent) states of a green fluorescent protein (GFP) mutant is demonstrated. A single-protein investigation shows the existence of two distinct bright states that display sharp two-photon cross-section bands peaked at 780 nm and at 870 nm. Fluorescence of these two species can be independently switched on and off. These results highlight a new photoconversion pathway for photochromic GFPs and can have significant applications in multi-photon confocal microscopy and in optical data-storage architectures.Keywords
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