Über die Sonderstellung der d-Glutaminsäure bei Versuchen mit d-Aminosäureoxydase. 7. Mitteilung über die Chemie der Tumoren

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Abstract
Most amino acids of the d-series are deaminated about 10 times as rapidly as their natural antipodes. However, d-glutamic acid, which is present in unusually high conc. in tumor tissue, is attacked very slowly. The course of the reaction is measured by the amt. of NH3 formed, as detd. by the method of Parnas. The only tissues able to bring about this oxidative deamination are kidney and liver. Lipmann and coworkers (Science, 91: 21, 1940) attempted to detn. the d-amino acid content of normal and tumor tissue acid hydrolyzates by the aid of enzyme preps. of d-amino acid oxidase. They found no difference between the conc. of d-glutamic acid in normal and tumor tissue, though they recovered added d-glutamic acid quantitatively from the hydrolyzates. Their difficulty was probably due to the use of crude enzyme preps. in the presence of a very complex substrate, and to the long duration of their expts. (20 hrs.). Kogl''s method, based on the isolation of the glutamic acids, is more reliable.

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