Selective biliary lipid secretion at low bile-salt-output rates in the isolated perfused rat liver. Effects of phalloidin
- 1 July 1986
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 237 (1) , 301-304
- https://doi.org/10.1042/bj2370301
Abstract
At high bile-salt-secretion rates the biliary secretion of phospholipids and cholesterol is dependent on that of the bile salts. However, at low bile-salt outputs some secretion remains. Isolated perfused rat livers were used in these experiments in order to study the bile-salt-independent secretion of biliary lipids. The livers were isolated and saline (0.9% NaCl), or phalloidin dissolved in saline, was added to the perfusion fluid after 1 h of liver isolation. The concentration and output of cholesterol was significantly decreased in phalloidin-treated livers compared with the controls, whereas there was no significant decrease in phospholipids; the secretion of cholesterol and phospholipids can thus be uncoupled from each other by the action of phalloidin. These experiments suggest that a proportion of cholesterol gets into bile independently of bile salts and phospholipids. These findings are discussed in relation to the supersaturation of some biles with cholesterol and its relationship to the bile-salt-independent fraction of cholesterol.This publication has 22 references indexed in Scilit:
- Evidence that a diminished bile acid pool precedes the formation of cholesterol gallstones in man.1973
- Bile Composition, Bile Salt Metabolism and GallstonesArchives of internal medicine (1960), 1972
- Biliary excretion of lecithin and cholesterol in the dogJournal of Clinical Investigation, 1972
- Micellar theory of biliary cholesterol excretionAmerican Journal of Physiology-Legacy Content, 1972
- The effect of bile salts upon lecithin synthesis.1971
- Diminished Bile Acid Pool Size in Patients with GallstonesGastroenterology, 1970
- GallstonesNew England Journal of Medicine, 1968
- [Determination of cholesterol in the bile of the rat by gas phase chromatography].1968
- Gluconeogenesis in the perfused rat liverBiochemical Journal, 1966
- A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATIONCanadian Journal of Biochemistry and Physiology, 1959