Synthesis of Newcastle disease virus polypeptides in a wheat germ cell-free system
- 1 April 1977
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 22 (1) , 97-101
- https://doi.org/10.1128/jvi.22.1.97-101.1977
Abstract
We have isolated 18S RNA from cytoplasmic extracts of Newcastle disease virus-infected Chinese hamster ovary cells and tested its ability to direct protein synthesis in extracts derived from wheat germ. The products of the cell-free reaction directed by this RNA contain polypeptides that comigrate with NP, M,F, and 47K roteins from virions. In addition, the products contain a polypeptide (67K) that migrates on polyacrylamide gels slightly faster than the HN protein from virions. Tryptic peptide analysis of the cell-free products and proteins from virions confirms their identity.This publication has 23 references indexed in Scilit:
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