Signalling downstream of activated mammalian Notch

28 September 1995

journal article
research article
Published by Springer Nature in Nature

Vol. 377 (6547) , 355-358
https://doi.org/10.1038/377355a0

Abstract

NOTCH belongs to a family of transmembrane proteins that are widely conserved from flies to vertebrates and are thought to be involved in cell-fate decisions. In Drosophila, the Suppressor of hairless (Su(H)) gene^1,2 and genes of the Enhancer of split (E(Spl)) complex, which encode proteins of the basic helix–loop–helix type^3,4 have been implicated in the Notch signalling pathway. Mammalian homologues of E(Spl), such as the mouse Hairy enhancer of split (HES-1 )⁵, have been isolated. Both HES-1 and the intracellular domain of murine Notch (mNotch) are able to block MyoD-induced myogenesis^5-7. Here we show that activated forms of mNotch associate with the human analogue of Su(H), KBF2/RBP-JK (refs 8, 9) and act as transcriptional activators through the KBF2-binding sites of the HES-1 promoter.

Keywords

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