Analisis Inmunoquimico de Extractos Solubles deParacoccidioides brasiliensis
- 1 January 1973
- journal article
- Published by Oxford University Press (OUP) in Medical Mycology
- Vol. 11 (1) , 80-88
- https://doi.org/10.1080/00362177385190171
Abstract
Agar gel electrophoresis of soluble extracts obtained from 9 cultures of Paracoccidioides brasiliensis, in alkaline pH, revealed 8 protein fractions, 5 aniodic and 3 cathiodic. Electrophoregrams revealed 15 enzymes, only 6 of which were further identified on immunoelectrophoregrams. Immunoelectrophoretic analysis of the extracts with hyperimmune serum prepared in rabbits confirmed that P. brasiliensis has a minimum of 25 antigenic fractions. One of them of cathodic mobility with alkaline phosphatase activity, reacted with all immune sera employed and thereby produced a characteristic antigen antibody system. An antibody corresponding to this fraction was formed early during the experimental infection of rabbits. This suggested that the antigen involved in the formation of arc E, was specific in human paracoccidioidomycosis. The existence of various antigenic fractions common to P. brasiliensis and species of Emmonsia was verified.Keywords
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