A Cell and Virus Disintegrator Using an Adjustable Conical Space
- 1 January 1973
- journal article
- research article
- Published by Taylor & Francis in Preparative Biochemistry
- Vol. 3 (3) , 243-256
- https://doi.org/10.1080/00327487308061509
Abstract
A simplified tissue disintegrator is described. The disruption of tissue is affected by forcing the suspended material through an adjustable conical space formed between a stationary cone in a moveable concavity. Compressed nitrogen at 50 Kg/cm2 appeared to be an adequate pressure for the disintegration of cells grown in tissue culture and for Ehrlich ascites tumour cells of mice. Influenza virus particles were also effectively disrupted by the procedure but at higher pressures. Tobacco Mosaic virus particles were broken down into short rods which, under pressure, became associated to form paracrystalline structures. However, cells provided with cell walls, such as yeast cells and Klebsiella organisms, could not be disintegrated in the apparatus.Keywords
This publication has 6 references indexed in Scilit:
- Removal of Anti-complementary Material and Australia Antigen from ImmunoglobulinPreparative Biochemistry, 1973
- Polyethylene glycol purification of influenza virus with respect to aggregation and antigenicityEpidemiology and Infection, 1972
- The disintegration of bacteria and other micro‐organisms by the M.S.E.‐Mullard Ultrasonic disintegratorJournal of Biochemical and Microbiological Technology and Engineering, 1961
- Observations on the use of ultrasound waves for the disruption of micro-organismsBiochimica et Biophysica Acta, 1959
- Some properties of the glutaminase of Clostridium welchiiBiochemical Journal, 1952
- II.—TISSUE DISINTEGRATORJournal of the Royal Microscopical Society, 1948