Induction and Rejoining of DNA Double-Strand Breaks in Normal Human Skin Fibroblasts after Exposure to Radiation of Different Linear Energy Transfer: Possible Roles of Track Structure and Chromatin Organization

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Abstract
Höglund, E. and Stenerlöw, B. Induction and Rejoining of DNA Double-Strand Breaks in Normal Human Skin Fibroblasts after Exposure to Radiation of Different Linear Energy Transfer: Possible Roles of Track Structure and Chromatin Organization. Radiat. Res. 155, 816–823 (2001). DNA double-strand breaks are nonrandomly induced by high-LET radiation. Differences in the induction and rejoining of DSBs after irradiation with ions having different LET were detected by fragment analysis. The data obtained indicate that the track structure of the traversing particle and its interaction with the different chromatin structures of the cellular DNA influence the yield as well as the distribution of the induced damage. The induction and rejoining of clustered DSBs induced by the same nitrogen ion fluence at LETs of 80–225 keV/μm were investigated by a detailed analysis of the DNA fragmentation patterns in normal human fibroblasts. The DSBs in the cells were allowed to rejoin during incubations for 0–20 h. Two s...